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ultrapure herring sperm dna solution (sheared)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ultrapure herring sperm dna solution (sheared)
    Ultrapure Herring Sperm Dna Solution (Sheared), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/herring+sperm+dna+solution/ultrapure+herring+sperm+dna+solution/pm40555232-202-54-60
    Average 90 stars, based on 1 article reviews
    ultrapure herring sperm dna solution (sheared) - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Structure-Activity Relationships of Bis-Intercalating Peptides and Their Application as Antibody-Drug Conjugate Payloads.
    Article Snippet: Synthetic analogs based on the DNA bis-intercalating natural product peptides sandramycin and quinaldopeptin were investigated as antibody drug conjugate (ADC) payloads.. Synthesis, biophysical characterization, and in vitro potency of 34 new analogs are described.. Conjugation of an initial drug-linker derived from a novel bis-intercalating peptide produced an ADC that was hydrophobic and prone to aggregation.

    Article Title: Method for biotechnological production of methylized cinnamic acids and cinnamic acid esters, methylized phenethylamines and the coupling products thereof, particularly of cinnamic acid amides
    Article Snippet: For the transformation of the plasmid DNA from Example 15 or 16, a main culture was first inoculated with 150 mL YPD medium (Formedium, Great Britain) from an overnight culture of the S. cerevisiae strain BY4741.

    Hybridization:

    Article Title: Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations
    Article Snippet: .. A hybridization mixture was prepared containing 500 ng DEEPER-Library DNA, 2 ug of RNA probes (1 ug from “+” clone transcripts and 1 ug from “−” clone transcripts, targeting Hg19 human exomes including −50 bp and +50 bp flanking intronic sequences, as well as 5′ and 3′ UTRs as described before), 7 ul Human Cot-1 DNA (ThermoFisher Scientific), 3 ul Herring Sperm DNA Solution (ThermoFisher Scientific), 10 μl blocking Oligos (1 nmol/ul each) with following sequences: Blocking Oligo 1: 5′-AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT-3′ Inverted dT Blocking Oligo 2: 5′-CCTCAGCAAGAGCACACGTCTGAACTCCAGTCAC-7 mer Index-ATCTCGTATGCCGTCTTCTGCTTG-3′ Inverted dT The hybridization mixture is heated for 5 minutes at 95 °C, then held at 67.5 °C. ..

    Article Title: Nucleic acid capture method
    Article Snippet: .. A hybridization mixture was prepared containing 500 ng DNA library, 2 ug of RNA probes (1 ug from “+” clone transcripts and 1 ug from “−” clone transcripts, targeting Hg19 human exomes including −50 bp and +50 bp flanking intronic sequences, as well as 5′ and 3′ UTRs as described before), 7 ul Human Cot-1 DNA (ThermoFisher Scientific), 3 ul Herring Sperm DNA Solution (ThermoFisher Scientific), 10 μl blocking Oligos (1 nmol/ul each) with following sequences: Blocking Oligo 1: 5′-AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG GTCGCCGTATCATT-3′ Inverted dT (SEQ ID NO. 913, where the last T is labelled with an inverted dT) Blocking Oligo 2: 5′-CCTCAGCAAGAGCACACGTCTGAACTCCAGTCAC-NN-NNNNN-ATCTCGTATGCCGTCTTCTGCTTG-3′ Inverted dT (SEQ ID NO. 914, where N can be any of A, T, C, and G, and the last G is labelled with an inverted dT) The hybridization mixture is heated for 5 minutes at 95° C., then held at 67.5° C. 25 ul pre-warmed (67.5° C.) 2.8× hybridization buffer (14×SSPE, 14×Denhardt's, 14 mM EDTA, 0.28% SDS) was added. ..

    Article Title: Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations.
    Article Snippet: .. A hybridization mixture was prepared containing 500 ng DEEPER-Library DNA, 2 ug of RNA probes (1 ug from “+” clone transcripts and 1 ug from “−” clone transcripts, targeting Hg19 human exomes including −50 bp and +50 bp flanking intronic sequences, as well as 5′ and 3′ UTRs as described before), 7 ul Human Cot-1 DNA (ThermoFisher Scientific), 3 ul Herring Sperm DNA Solution (ThermoFisher Scientific), 10 μl blocking Oligos (1 nmol/ul each) with following sequences: Blocking Oligo 1: 5′-AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT-3′ Inverted dT Blocking Oligo 2: 4Scientific RepoRts | 7: 3356 | DOI:10.1038/s41598-017-03448-8 5 ′-CCTCAGCAAGAGCACACGTCTGAACTCCAGTCAC-7 mer Index-ATCTCGTATGCC GTCTTCTGCTTG-3′ Inverted dT The hybridization mixture is heated for 5 minutes at 95 °C, then held at 67.5 °C. ..

    Blocking Assay:

    Article Title: Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations
    Article Snippet: .. A hybridization mixture was prepared containing 500 ng DEEPER-Library DNA, 2 ug of RNA probes (1 ug from “+” clone transcripts and 1 ug from “−” clone transcripts, targeting Hg19 human exomes including −50 bp and +50 bp flanking intronic sequences, as well as 5′ and 3′ UTRs as described before), 7 ul Human Cot-1 DNA (ThermoFisher Scientific), 3 ul Herring Sperm DNA Solution (ThermoFisher Scientific), 10 μl blocking Oligos (1 nmol/ul each) with following sequences: Blocking Oligo 1: 5′-AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT-3′ Inverted dT Blocking Oligo 2: 5′-CCTCAGCAAGAGCACACGTCTGAACTCCAGTCAC-7 mer Index-ATCTCGTATGCCGTCTTCTGCTTG-3′ Inverted dT The hybridization mixture is heated for 5 minutes at 95 °C, then held at 67.5 °C. ..

    Article Title: Nucleic acid capture method
    Article Snippet: .. A hybridization mixture was prepared containing 500 ng DNA library, 2 ug of RNA probes (1 ug from “+” clone transcripts and 1 ug from “−” clone transcripts, targeting Hg19 human exomes including −50 bp and +50 bp flanking intronic sequences, as well as 5′ and 3′ UTRs as described before), 7 ul Human Cot-1 DNA (ThermoFisher Scientific), 3 ul Herring Sperm DNA Solution (ThermoFisher Scientific), 10 μl blocking Oligos (1 nmol/ul each) with following sequences: Blocking Oligo 1: 5′-AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG GTCGCCGTATCATT-3′ Inverted dT (SEQ ID NO. 913, where the last T is labelled with an inverted dT) Blocking Oligo 2: 5′-CCTCAGCAAGAGCACACGTCTGAACTCCAGTCAC-NN-NNNNN-ATCTCGTATGCCGTCTTCTGCTTG-3′ Inverted dT (SEQ ID NO. 914, where N can be any of A, T, C, and G, and the last G is labelled with an inverted dT) The hybridization mixture is heated for 5 minutes at 95° C., then held at 67.5° C. 25 ul pre-warmed (67.5° C.) 2.8× hybridization buffer (14×SSPE, 14×Denhardt's, 14 mM EDTA, 0.28% SDS) was added. ..

    Article Title: Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations.
    Article Snippet: .. A hybridization mixture was prepared containing 500 ng DEEPER-Library DNA, 2 ug of RNA probes (1 ug from “+” clone transcripts and 1 ug from “−” clone transcripts, targeting Hg19 human exomes including −50 bp and +50 bp flanking intronic sequences, as well as 5′ and 3′ UTRs as described before), 7 ul Human Cot-1 DNA (ThermoFisher Scientific), 3 ul Herring Sperm DNA Solution (ThermoFisher Scientific), 10 μl blocking Oligos (1 nmol/ul each) with following sequences: Blocking Oligo 1: 5′-AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT-3′ Inverted dT Blocking Oligo 2: 4Scientific RepoRts | 7: 3356 | DOI:10.1038/s41598-017-03448-8 5 ′-CCTCAGCAAGAGCACACGTCTGAACTCCAGTCAC-7 mer Index-ATCTCGTATGCC GTCTTCTGCTTG-3′ Inverted dT The hybridization mixture is heated for 5 minutes at 95 °C, then held at 67.5 °C. ..



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